Lab 6: Biological Chemicals, Nutrition, and Health 

Materials

• Part I
• Carbohydrates 
• test tube rack
• hot plate
• 400 mL beaker
• 3 test tubes
• onion juice
• potato juice
• Benedict's Reagent
• distilled water
• Lugol's Iodine 
• Fats and Lipids

• Sudan III
• extracts of flour, cream, coconut
• margarine
• filter paper
• pencils
• dedicated Pasteur Pipet
• water bath 
• Proteins
• Buiret's Agent
• egg white
• chicken broth 
• distilled water
• test tubes
• test tube rack
• CuSO4
• wax pencils 
• Common Foods for Testing 
• gelatin
• lite and regular pancake syrup
• instant and ground coffee
• honey
• olive oil
• peanut oil
• candy
• Part II
• digital blood pressure monitor 
• finger pulse oximeter

Exercises

• Main Idea:
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• Test the major food groups (fats, lipids, and proteins) 
• Test cardiovascular system
• blood pressure
• pulse
• oxygen saturation
• experiments 
• Part I
• Carbohydrates
• Use Benedict's test for simple sugars
• Test presence or absence of simple sugars in the solutions 
• Mark tubes with a wax pencil 
• Tube 1) onion juice to 1 cm mark, Benedict's Reagent to 3 cm mark
• Tube 2) potato juice to 1 cm mark, Benedict's reagent to 3 cm mark 
• Tube 3) water to 1 cm mark, Benedict's reagent to 3 cm mark 
• Heat tubes in boiling water for 3 minutes 
• Observe color changes 
• Repeat with Lugol's Iodine
• Fats and Lipids
• Mark filter paper with pencil for each food type
• Blot the extracts on a piece of filter paper 
• Allow to dry
• Soak paper in Sudan III for 3 minutes 
• Remove paper and rinse in water for 1 minute 
• Determine which substance has fats by the stains
• Proteins
• Fill test tubes
• Tube 1) 1 drop egg white, 1 drop Biuret's Reagent
• Tube 2) 1 drop chicken broth, 1 drop Biuret's Reagent
• Tube 3) 1 drop water, 1 drop Biuret's Reagent 
• Test color changes 
• Test unknowns 
• Part II
• Take vital signs for resting rate of lab partner using digital blood pressure monitor 
• Record data
• Use Finger Pulse Oximeter to take heart rate for lab partner 
• Do an experiment that would change blood pressure
• Take blood pressure again and record results