Lab 8: Gel Electrophoresis and Proteins
Materials
- Parts I and II:
- Procell electrophoresis apparatus, gel molds, combs, Power units, buffer, gels
- Protein production through Transcription and Translation
Exercises
- Main Idea:
- Perform starch gel electrophoresis
- Part I
- Fill wells and run the bells at 1 hour at 170 volts
- While gels are running, examine slides of normal and sickle cells
- Compare shapes of cells
- Make drawings
- Part II
- Open the file
- Go to formula
- Fill in table of nitrogenous bases
Lab 7: Genetic Architecture and Karyology
Materials
- Parts I and II:
- Parts of body for Rebop
- Computer
Exercises
- Main Idea:
- Study relationship between genotype and phenotype by building fictional organisms ("Rebop")
- Part I
- Choose two alleles per character
- Use table to determine which genes you have and how they will be expressed in phenotype
- Build the Rebop based on chosen genes
- Part II
- use slide of chromosome to match homologous chromosomes using mouse
Lab 6: Biological Chemicals, Nutrition, and Health
Materials
- Part I
- Carbohydrates
- test tube rack
- hot plate
- 400 mL beaker
- 3 test tubes
- onion juice
- potato juice
- Benedict's Reagent
- distilled water
- Lugol's Iodine
- Fats and Lipids
- Sudan III
- extracts of flour, cream, coconut
- margarine
- filter paper
- pencils
- dedicated Pasteur Pipet
- water bath
- Proteins
- Buiret's Agent
- egg white
- chicken broth
- distilled water
- test tubes
- test tube rack
- CuSO4
- wax pencils
- Common Foods for Testing
- gelatin
- lite and regular pancake syrup
- instant and ground coffee
- honey
- olive oil
- peanut oil
- candy
- Part II
- digital blood pressure monitor
- finger pulse oximeter
Exercises
- Main Idea:
- Test the major food groups (fats, lipids, and proteins)
- Test cardiovascular system
- blood pressure
- pulse
- oxygen saturation
- experiments
- Part I
- Carbohydrates
- Use Benedict's test for simple sugars
- Test presence or absence of simple sugars in the solutions
- Mark tubes with a wax pencil
- Tube 1) onion juice to 1 cm mark, Benedict's Reagent to 3 cm mark
- Tube 2) potato juice to 1 cm mark, Benedict's reagent to 3 cm mark
- Tube 3) water to 1 cm mark, Benedict's reagent to 3 cm mark
- Heat tubes in boiling water for 3 minutes
- Observe color changes
- Repeat with Lugol's Iodine
- Fats and Lipids
- Mark filter paper with pencil for each food type
- Blot the extracts on a piece of filter paper
- Allow to dry
- Soak paper in Sudan III for 3 minutes
- Remove paper and rinse in water for 1 minute
- Determine which substance has fats by the stains
- Proteins
- Fill test tubes
- Tube 1) 1 drop egg white, 1 drop Biuret's Reagent
- Tube 2) 1 drop chicken broth, 1 drop Biuret's Reagent
- Tube 3) 1 drop water, 1 drop Biuret's Reagent
- Test color changes
- Test unknowns
- Part II
- Take vital signs for resting rate of lab partner using digital blood pressure monitor
- Record data
- Use Finger Pulse Oximeter to take heart rate for lab partner
- Do an experiment that would change blood pressure
- Take blood pressure again and record results
Lab 5: Cell Division and Mitosis
Materials
- Parts I, II and III:
- Whole mount slides
- Microscope
- Onion root
- Stain
- Pipe cleaners
- Plastic rings
Exercises
- Main Idea:
- Part I
- Examine the whole mount slides and find different phases of mitosis
- Part II
- Prepare onion slides by staining the cells
- Find stages of mitosis under the microscope
- Answer questions in packet
- Part III
- Create pipe cleaner model that represent homologous chromosomes
- Bind "homologous chromosomes" together
- Go through the phases with the models
